Professor, Chemistry
Professor, Biological Sciences
Director, Alabama High Field NMR Lab
office: MSB 311
lab: MSB 300, 306, 308
phone: (256) 824-2477
fax: (256) 824-6349
email: John Shriver
Research and Teaching Webpage
Résumé
B.A. Biology, West Virginia University, 1971
Ph.D. Chemistry, Case Western Reserve University, 1977
Post-doctoral Fellow, University of Alberta, 1977-81.
Professor, Medical Biochemistry, Southern Illinois University School of Medicine, 1981-2001
Professor, UA Huntsville, 2001-present
Teaching Interests
Biophysical chemistry, Biochemistry
Research Interests
My laboratory is devoted to characterizing the energetics of protein folding and ligand binding with a special emphasis on protein-DNA interactions that are essential in many normal cellular functions such as gene regulation, expression, recombination, and chromatin organization. We take advantage of the high stability of DNA binding proteins from hyperthermophiles such as Sulfolobus to obtain unusually precise and accurate data that are difficult to obtain on less stable mesophile proteins.
Selected Publications
Structure, Stability, and Flexibility of Ribosomal Protein L14e from Sulfolobus acidocaldarius, S.P. Edmondson, J. Turri, K. Smith, A.T. Clark, and J.W. Shriver, Biochemistry 48, 5553-62 (2009). (Pubmet abstract)
Ligand Binding Interactions and Stability, John W. Shriver and Stephen Edmndson, Methods. Mol. Biol. 490, 135-64 (2009). (Pubmed abstract)
Defining the Stability of Multimeric Proteins, John W. Shriver and Stephen P. Edmondson, Methods Mol. Biol. 490, 57-82 (2009). (Pubmed abstract)
Carboxyl pKa values, ion pairs, hydrogen bonding, and the pH-dependence of folding the hyperthermophile proteins Sac7d and Sso7d, A.T. Clark, K. Smith, R. Muhandidram, S.P. Edmondson, and J.W. Shriver, J. Mol. Biol. 372, 992-1008 (2007). (Pubmed abstract)
Solution Structure, Stability, and Nucleic Acid Binding of the Hyperthermophile Protein Sso10b2, K. Biyani, M.A. Kahsai, A.T. Clark, T.L. Armstrong, S.P. Edmondson, and J.W. Shriver, Biochemistry 44, 14217-30 (2005). (Pubmed abstract)
Stability and Flexibility in the Structure of the Hyperthermophile DNA-binding Protein Sac7d, M.A. Kahsai, E. Martin, S.P. Edmondson, and J.W. Shriver, Biochemistry 44, 13500-9 (2005). (Pubmet abstract)
Thermodynamics of DNA Binding and Distortion by the Hyperthermophile Chromatin Protein Sac7d, W.B. Peters, S.P. Edmondson, and J.W. Shriver, J. Mol Biol. 34, 339-60 (2004). (Pubmet abstract)
Thermodynamics of Core Hydrophobicity and Packing in the Hyperthermophile Proteins Sac7d and Sso7d, A.T. Clark, B.S. McCrary, S.P. Edmondson, and J. W. Shriver, Biochemistry 43, 2840-53 (2004). (Pubmed abstract)