Research Assistant Professor, Chemistry
office: MSB 225
phone: (256) 824-6935
fax: (256) 824-6349
email: Pam Twigg
Résumé
B.S. Chemical Engineering, Auburn University, 1982
M.S. Biomedical Engineering, University of Alabama at Birmingham, 1986
Ph.D. Molecular Biophysics, Florida State University, 2001
Research Assistant Professor, UA Huntsville, 2004 – present
Research Interests
My research focus is on the structural and biophysical characterization of proteins, using methods such as x-ray crystallography, NMR spectroscopy, circular dichroism, calorimetry, and fluorescence spectroscopy. In particular, I am interested in the protein-protein interactions and function of proteins related to the pathology of Huntington’s Disease, a genetic movement disorder.
Selected Publications
• Wilson, R. C., Hughes, R. C., Flatt, J. W., Meehan, E. J., Ng, J. D., and Twigg, P. D. 2009. “The crystal structure of full-length ubiquitin-conjugating enzyme E2-25K (Huntingtin-interacting protein 2).” Acta Cryst. F, accepted for publication.
• Wilson, R. C., Hughes, R. C., Curto, E. V., Ng, J. D., and Twigg, P. D. 2007. “Backbone 1H, 15N, and 13C Resonance Assignments and Secondary Structure of a Novel Protein OGL-20Pt-358 from Hyperthermophile Thermococcus thioreducens sp. Nov.” Molecules and Cells, 24(3), 437-440.
• Twigg, P. D., G. Parthasarathy, L. Guerrero, T. M. Logan, and D. L. D. Caspar. 2001. “Disordered to Ordered Folding in the Regulation of Diphtheria Toxin Repressor Activity.” Proc. Natl. Acad. Sci. USA, 98, 11259-64.
• Wang, G., G. P. Wylie, P. D. Twigg, D. L. D. Caspar, J. R. Murphy, and T. M. Logan. 1999. “Solution Structure and Peptide Binding Studies of the C-Terminal SH3-Like Domain of the Diphtheria Toxin Repressor Protein.” Proc. Natl. Acad. Sci. USA, 96, 6119-6124.
• Twigg, P. D., G. P. Wylie, G. Wang, D. L. D. Caspar, J. R. Murphy, and T. M. Logan. 1999. “Expression and Assignment of the 1H, 15N, and 13C Resonances of the C-terminal Domain of the Diphtheria Toxin Repressor.” J. Biomolecular NMR, 13(2), 197-198.
• Wardell, M. R., Skinner, R., Carter, D. C., Twigg, P. D., & Abrahams, J.-P. 1997. “Improved diffraction of antithrombin crystals grown in microgravity.” Acta Cryst.D, 53, 622-625.
• Ho, J.X., E.W. Holowachuk, E.J. Norton, P.D. Twigg, and D.C. Carter. 1993. “X-ray and Primary Structure of Horse Serum Albumin (Equus caballus) at 0.27-nm Resolution.” Eur. J. Biochem., 215, 205-212.
• Carter, D.C., He, X.-M., Munson, S.H., Twigg, P.D., Gernert, K.M., Broom, M.B., and Miller, T.Y. 1989. “Three-Dimensional Structure of Human Serum Albumin.” Science, 244, 1195-1198.